The Jonah Lomu signed jersey was given a pre-sale estimation price of between $3,000 and $4,000 (£1,635 - £2,180).
Conveniently falling on the same day as Crowe's 54th birthday and would-be 15th wedding anniversary, the bidding paused to bring out local singer Alisa Nasteski to serenade the star with "Happy Birthday".
A replica Roman chariot from the same movie sold for $65,000, while a wooden training sword was won for $20,000 and an aluminium prop sword went for $70,000.
"People just went mad".
"It's been a lot of fun putting it together for you", he told the crowd at the inner-city Carriageworks building.
"Not a bad hourly rate for a 5 hour shift", Crowe tweeted, adding there was a "bunch of stuff" he didn't really want to sell coming home.
Chief executive Gary Singer said items linked to the film were the most popular.More news: CL: Juventus can beat Real Madrid in double header, says Gianluigi Buffon
Two items from Crowe's earlier film days piqued the interest of the National Museum Australia director Mathew Trinca.
A second bat used by Martin Crowe to score 142 test runs a century against England at Lords Cricket ground in 1994 also sold for $21,000.
A stunt cuirass worn by Russell Crowe in the scene depicting the death of character Maximus in the film Gladiator sold for a staggering $120,000 - $100,000 more than its estimated value.
While six-figure sums were dropped for a handful of artworks - including $360,000 for a 1960 Charles Blackman - other items were far less orthodox.
More surprisingly, a costume that Crowe wore in the same 2003 movie wasn't far behind, raking in AU$115,000.
Music lovers in attendance were catered for with the collection of high-end guitars on offer, and also the 1986 Grammy Award which was jointly presented to Johnny Cash, Carl Perkins, Jerry Lee Lewis, Roy Orbison, Sam Phillips, Rick Nelson and Chips Mosman which sold for $150,000. It would receive a 22 percent commission, which the buyers have to fork out on top of the bid money.
Perhaps the strangest item sold was a used jockstrap that Crowe wore while portraying boxer James J. Braddock in "Cinderella Man", which wound up selling for a surprising $7,000.